Park C M, Lopinski J D, Masuda J, Tzeng T H, Bruenn J A
Department of Biological Sciences, State University of New York at Buffalo, 14260, USA.
Virology. 1996 Feb 15;216(2):451-4. doi: 10.1006/viro.1996.0083.
Two double-stranded RNA viruses exist as permanent persistent infections of the yeast Saccharomyces cerevisiae: ScVL1 and ScVLa. Both belong to the Totiviridae, which include a number of fungal and protozoan double-stranded RNA viruses. Although ScVL1 and ScVLa share the same genomic organization and mode of expression and coexist in the same cells, they show no evidence of recombination: with one limited exception, sequence conservation is detectable only in regions conserved in all totiviruses. Both have two open reading frames on their single essential RNAs: cap (encoding a capsid polypeptide) and pol (encoding an RNA-dependent RNA polymerase). The ScVLa virus, like ScVL1, appears to express its Pol domain by a -1 translational frameshift.
有两种双链RNA病毒以永久性持续感染的形式存在于酿酒酵母中：ScVL1和ScVLa。它们都属于双病毒科，该科包括许多真菌和原生动物双链RNA病毒。尽管ScVL1和ScVLa具有相同的基因组结构和表达模式，且共存于同一细胞中，但它们没有重组的迹象：只有一个有限的例外情况，序列保守性仅在所有双病毒科病毒中保守的区域才能检测到。它们的单一必需RNA上都有两个开放阅读框：cap（编码衣壳多肽）和pol（编码RNA依赖性RNA聚合酶）。ScVLa病毒与ScVL1一样，似乎通过-1移码翻译来表达其Pol结构域。
