Zhang W, Reichlin M
Oklahoma Medical Research Foundation and the College of Medicine, The University of Oklahoma Health Sciences Center Oklahoma City, 73104, USA.
Arthritis Rheum. 1996 Mar;39(3):522-31. doi: 10.1002/art.1780390321.
To determine the relationship of anti-Ro/SS-A and anti-La/SS-B antibodies to anti-double- stranded DNA (anti-dsDNA) in sera from patients with systemic lupus erythematosus.
Sera with anti-Ro/SS-A alone (n = 5) or those with anti-Ro/SS-A and anti-La/SS-B (n = 7) were absorbed with purified Ro/SS-A and La/SS-B, respectively. The absorbed sera were then tested for reactivity with MOLT-4 extract by Western blot and dsDNA by enzyme-linked immunosorbent assay (ELISA). With selected sera, anti-dsDNA was isolated on DNA cellulose columns and anti-Ro/SS-A and anti-La/SS-B were isolated on antigen-affinity columns. Reactivity between anti-dsDNA and autologous anti-Ro/SS-A or anti-La/SS-B, as well as inhibition by cognate antigens, was studied.
After absorption, all sera showed reactivity with small nuclear RNP A and D bands in Western blots, and some showed reactivity with dsDNA by ELISA. Anti-dsDNA populations (n = 4) were purified on dsDNA cellulose columns. Anti-Ro/SS-A (n = 1) and anti-La/SS-B (n = 3) were affinity purified from the same sera as the anti-dsDNA. In all cases, anti-dsDNA bound autologous anti-Ro/SS-A and anti-La/SS-B much more strongly than it bound normal pooled IgG. Moreover, dsDNA, but not RNA, blocked these interactions. In addition, Ro/SS-A blocked anti-Ro/SS-A and La/SS-B blocked anti-La/SS-B in these same interactions.
In sera with anti-Ro/SS-A and anti- La/SS-B, there are subpopulations of these antibodies that bind and mask anti-dsDNA. We hypothesize that these anti-Ro/SS-A and anti-La/SS-B antibodies are antiidiotypes to idiotypes on anti-dsDNA and that they both mask and down-regulate these anti-dsDNA antibodies.
确定系统性红斑狼疮患者血清中抗Ro/SS-A和抗La/SS-B抗体与抗双链DNA（抗dsDNA）之间的关系。
分别用纯化的Ro/SS-A和La/SS-B吸附单独含有抗Ro/SS-A的血清（n = 5）或同时含有抗Ro/SS-A和抗La/SS-B的血清（n = 7）。然后通过蛋白质印迹法检测吸附后的血清与MOLT-4提取物的反应性，并通过酶联免疫吸附测定（ELISA）检测与dsDNA的反应性。对于选定的血清，在DNA纤维素柱上分离抗dsDNA，在抗原亲和柱上分离抗Ro/SS-A和抗La/SS-B。研究抗dsDNA与自身抗Ro/SS-A或抗La/SS-B之间的反应性以及同源抗原的抑制作用。
吸附后，所有血清在蛋白质印迹中均显示与小核核糖核蛋白A和D带反应，部分血清通过ELISA显示与dsDNA反应。在dsDNA纤维素柱上纯化了抗dsDNA群体（n = 4）。从与抗dsDNA相同的血清中亲和纯化了抗Ro/SS-A（n = 1）和抗La/SS-B（n = 3）。在所有情况下，抗dsDNA与自身抗Ro/SS-A和抗La/SS-B的结合比与正常混合IgG的结合要强得多。此外，dsDNA而非RNA可阻断这些相互作用。另外，在这些相同的相互作用中，Ro/SS-A可阻断抗Ro/SS-A，La/SS-B可阻断抗La/SS-B。
在含有抗Ro/SS-A和抗La/SS-B的血清中，存在这些抗体的亚群，它们结合并掩盖抗dsDNA。我们推测这些抗Ro/SS-A和抗La/SS-B抗体是抗dsDNA独特型的抗独特型抗体，它们既能掩盖又能下调这些抗dsDNA抗体。
