Zhao B, Shen J, Li M, Li M, Wan Q, Xin W
Institute of Biophysics, Academia Sinica, Beijing, China.
Biochim Biophys Acta. 1996 Mar 1;1315(2):131-7. doi: 10.1016/0925-4439(95)00112-3.
The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.
采用电子自旋共振（ESR）和化学发光技术研究了奎宁对一氧化氮（NO）和氧自由基的清除作用及其对心肌缺血再灌注损伤的保护作用。奎宁能有效抑制过氧亚硝酸根（ONOO-）的氧化活性（IC50 = 7×10（-5）mmol/L），并清除黄嘌呤与黄嘌呤氧化酶反应产生的氧自由基（IC50 = 2/5×10（-4）mmol/L）。很难找到另一种抗氧化剂能同时如此有效地清除ONOO-和氧自由基。在心肌缺血再灌注损伤体系中，奎宁能同时清除缺血再灌注心肌产生的NO和氧自由基，并降低缺血再灌注心脏冠状动脉流出液中乳酸脱氢酶（LDH）和肌酸激酶（CK）的活性，从而保护心脏免受缺血再灌注损伤。0.1 mmol/L奎宁的保护作用与1500 U/ml超氧化物歧化酶（SOD）和过氧化氢酶的保护作用相似。
