奎宁对一氧化氮和氧自由基的清除作用及其对心肌缺血再灌注损伤的保护作用。
Scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on the myocardium from the injury of ischemia-reperfusion.
作者信息
Zhao B, Shen J, Li M, Li M, Wan Q, Xin W
机构信息
Institute of Biophysics, Academia Sinica, Beijing, China.
出版信息
Biochim Biophys Acta. 1996 Mar 1;1315(2):131-7. doi: 10.1016/0925-4439(95)00112-3.
The scavenging effect of Chinonin on NO and oxygen free radicals and its protective effect on myocardium from the ischemia-reperfusion injury was studied with electron spin resonance (ESR) and chemiluminescence techniques. Chinonin can effectively inhibit the oxidative activity of ONOO-, (the IC50 = 7 x 10 (-5) mmol/L) and scavenge oxygen free radicals generated from the reaction of xanthine and xanthine oxidase (the IC50 = 2/5 x 10(-4) mmol/l). It is difficult to find another antioxidant which can scavenge so effectively both ONOO- and oxygen free radicals simultaneously. In the system of ischemia-reperfusion injury of myocardium, Chinonin can, in parallel, scavenge the NO and oxygen free radicals generated from the ischemia-reperfused myocardium, and decrease the activities of lactate dehydrogenase (LDH) and creatine kinase (CK) in the coronary artery effluent of ischemia-reperfused heart and therefore protect the heart from ischemia-reperfusion injury. The protective effect of 0.1 mmol/l Chinonin is similar to that of 1500 U/ml SOD and catalase.
采用电子自旋共振（ESR）和化学发光技术研究了奎宁对一氧化氮（NO）和氧自由基的清除作用及其对心肌缺血再灌注损伤的保护作用。奎宁能有效抑制过氧亚硝酸根（ONOO-）的氧化活性（IC50 = 7×10（-5）mmol/L），并清除黄嘌呤与黄嘌呤氧化酶反应产生的氧自由基（IC50 = 2/5×10（-4）mmol/L）。很难找到另一种抗氧化剂能同时如此有效地清除ONOO-和氧自由基。在心肌缺血再灌注损伤体系中，奎宁能同时清除缺血再灌注心肌产生的NO和氧自由基，并降低缺血再灌注心脏冠状动脉流出液中乳酸脱氢酶（LDH）和肌酸激酶（CK）的活性，从而保护心脏免受缺血再灌注损伤。0.1 mmol/L奎宁的保护作用与1500 U/ml超氧化物歧化酶（SOD）和过氧化氢酶的保护作用相似。
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