Sompayrac L, Jane S, Danna K J
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309, USA.
Virology. 1996 Apr 15;218(2):412-6. doi: 10.1006/viro.1996.0212.
We have used mRNA differential display to search for cellular genes whose expression is modulated in SV40-transformed cells. We identified a gene, the alpha1 subunit of type XI procollagen (alpha1(XI)), whose mRNA level is reduced in most F111 rat cell lines that express either wild-type SV40 T antigen or an amino-terminal fragment of T antigen (T147D). alpha1(XI) mRNA expression is also reduced in rat embryo fibroblasts transformed by SV40. This phenomenon is not unique to rat cells, because alpha1(XI) mRNA expression is repressed in C3H10T1/2 mouse cells transformed by either T147D or wild-type SV40. Interestingly, alpha1(XI) mRNA levels are not reduced in F111 cells that express only the polyomavirus large T antigen, but are reduced in F111 cells transformed by polyoma virus (which expresses all three polyomavirus T antigens) and in F111 cells that express the adenovirus E1A proteins. Thus, repression of alpha1(XI) mRNA expression may be a common feature of transformation by DNA tumor viruses.
我们利用mRNA差异显示技术来寻找在SV40转化细胞中表达受到调控的细胞基因。我们鉴定出一个基因，即XI型前胶原的α1亚基（α1(XI)），在大多数表达野生型SV40 T抗原或T抗原氨基末端片段（T147D）的F111大鼠细胞系中，其mRNA水平降低。在被SV40转化的大鼠胚胎成纤维细胞中，α1(XI) mRNA表达也降低。这种现象并非大鼠细胞所特有，因为在被T147D或野生型SV40转化的C3H10T1/2小鼠细胞中，α1(XI) mRNA表达也受到抑制。有趣的是，仅表达多瘤病毒大T抗原的F111细胞中α1(XI) mRNA水平并未降低，但在被多瘤病毒转化的F111细胞（该病毒表达所有三种多瘤病毒T抗原）和表达腺病毒E1A蛋白的F111细胞中，α1(XI) mRNA水平降低。因此，α1(XI) mRNA表达的抑制可能是DNA肿瘤病毒转化的一个共同特征。
