Cavaillé F, Kacémi A, Mondon F, Fournier T, Ferré F
Institut National de la Santé et de la Recherche Médicale, Paris, France.
Am J Obstet Gynecol. 1995 Dec;173(6):1793-9. doi: 10.1016/0002-9378(95)90429-8.
Our purpose was to compare the protein isoform composition of the contractile apparatus at different levels of the fetoplacental vessel musculature at term.
Umbilical, chorionic, and stem villi vessel protein extracts were run on one- and two-dimensional gel electrophoresis; previously characterized human myometrium proteins were used as the smooth muscle proteins of reference.
Fetoplacental vessel musculature exhibited a high actin/myosin ratio. The presence, in varying quantities, of myosin heavy chain and actin isoforms of smooth muscle type in the different vessels reflected their degree of differentiation. The presence of nonmuscle protein isoforms, particularly in stem villi vessels, indicated a certain degree of immaturity.
The presence of smooth muscle contractile protein isoforms indicates that fetoplacental vessel musculature is highly differentiated. Regional modulation of fetoplacental blood flow could be, in part, the result of local differences in contractile apparatus protein composition.
我们的目的是比较足月时胎儿胎盘血管肌肉组织不同水平收缩装置的蛋白质同工型组成。
脐血管、绒毛膜血管和绒毛干血管的蛋白质提取物进行一维和二维凝胶电泳；先前已鉴定的人子宫肌层蛋白质用作平滑肌蛋白质的参照。
胎儿胎盘血管肌肉组织表现出较高的肌动蛋白/肌球蛋白比率。不同血管中平滑肌型肌球蛋白重链和肌动蛋白同工型数量各异，反映了它们的分化程度。非肌肉蛋白质同工型的存在，尤其是在绒毛干血管中，表明存在一定程度的不成熟。
平滑肌收缩蛋白同工型的存在表明胎儿胎盘血管肌肉组织高度分化。胎儿胎盘血流的区域调节可能部分是收缩装置蛋白质组成局部差异的结果。
