Weiler S R, Mou S, DeBerry C S, Keller J R, Ruscetti F W, Ferris D K, Longo D L, Linnekin D
Division of Cancer Treatment, and Biological Carcinogenesis and Development Program, SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
Blood. 1996 May 1;87(9):3688-93.
Stem cell factor (SCF) is a hematopoietic growth factor that interacts with the receptor tyrosine kinase, c-kit. We have found that SCF-stimulates rapid and transient tyrosine phosphorylation of JAK2 in human and murine cell lines, as well as in normal human progenitor cells. JAK2 and c-kit were associated in unstimulated cells with further recruitment of JAK2 to the c-kit receptor complex after SCF stimulation. Treatment of cells with JAK2 antisense oligonucleotides resulted in a 46% decrease in SCF-induced proliferation. These data demonstrate that SCF induces tyrosine phosphorylation of JAK2 and suggest that JAK2 is a component of the SCF signal transduction pathway.
干细胞因子（SCF）是一种造血生长因子，它与受体酪氨酸激酶c-kit相互作用。我们发现，SCF可刺激人源和鼠源细胞系以及正常人祖细胞中JAK2的快速和瞬时酪氨酸磷酸化。在未受刺激的细胞中，JAK2与c-kit相关联，SCF刺激后JAK2会进一步募集到c-kit受体复合物中。用JAK2反义寡核苷酸处理细胞会导致SCF诱导的增殖减少46%。这些数据表明，SCF可诱导JAK2的酪氨酸磷酸化，并提示JAK2是SCF信号转导途径的一个组成部分。
