Kimura M, Ikeda T, Fukumoto D, Yamasaki N, Yonekura M
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 1995 Dec;59(12):2328-9. doi: 10.1271/bbb.59.2328.
The complete amino acid sequence of a proteinaceous cysteine proteinase inhibitor from the fruit of avocado (avocado cystatin) is presented. The protein consists of 100 amino acid residues and has a molecular mass of 11,300 Da. Comparison of this sequence with sequences of plant cysteine proteinase inhibitors (phytocystatins), including oryzacystatins I and II from rice seeds, cowpea cystatin, and corn cystatin, showed that the avocado cystatin molecule has 60% and 54% residues identical with the two forms of the rice seed proteins, oryzacystatins I and II, respectively, and 64% and 63% with the cowpea and corn proteins, respectively. The totally conserved sequence, Gln-Val-Val-Ala-Gly, among several of the animal cystatins as well as phytocystatins, is at positions 47-51 in the avocado cystatin molecule.
本文给出了鳄梨果实中一种蛋白质类半胱氨酸蛋白酶抑制剂（鳄梨半胱氨酸蛋白酶抑制剂）的完整氨基酸序列。该蛋白质由100个氨基酸残基组成，分子量为11300道尔顿。将此序列与植物半胱氨酸蛋白酶抑制剂（植物半胱氨酸蛋白酶抑制剂）的序列进行比较，包括水稻种子中的水稻半胱氨酸蛋白酶抑制剂I和II、豇豆半胱氨酸蛋白酶抑制剂和玉米半胱氨酸蛋白酶抑制剂，结果表明，鳄梨半胱氨酸蛋白酶抑制剂分子与水稻种子蛋白的两种形式水稻半胱氨酸蛋白酶抑制剂I和II分别有60%和54%的残基相同，与豇豆和玉米蛋白分别有64%和63%的残基相同。在几种动物半胱氨酸蛋白酶抑制剂以及植物半胱氨酸蛋白酶抑制剂中完全保守的序列Gln-Val-Val-Ala-Gly，位于鳄梨半胱氨酸蛋白酶抑制剂分子的第47-51位。
