Sessink P J, Vaes W H, van den Broek P H, Noordhoek J, Bos R P
Department of Toxicology, Faculty of Medical Sciences, University of Nijmegen, Netherlands.
J Chromatogr B Biomed Appl. 1995 Nov 17;673(2):205-12. doi: 10.1016/0378-4347(95)00264-7.
Sensitive methods for the determination of the cyclophosphamide metabolites nornitrogen mustard, 4-ketocyclophosphamide and carboxyphosphamide are presented. After liquid-liquid extraction and derivatization, the metabolites are determined by gas chromatography and thermionic specific detection. The methods were used to study the in vitro biotransformation of cyclophosphamide with S-9 liver fractions of human donors. The results show large interindividual differences in the formation of nornitrogen mustard and carboxyphosphamide. 4-Ketocyclophosphamide was not detected.
本文介绍了测定环磷酰胺代谢产物去甲氮芥、4-酮环磷酰胺和羧基磷酰胺的灵敏方法。经过液-液萃取和衍生化后，采用气相色谱法和热离子特异性检测法测定这些代谢产物。这些方法用于研究环磷酰胺在人供体肝脏S-9组分存在下的体外生物转化。结果表明，去甲氮芥和羧基磷酰胺的生成存在较大的个体差异。未检测到4-酮环磷酰胺。
