Wun T C, Bittman R
Biochemistry. 1977 May 17;16(10):2080-6. doi: 10.1021/bi00629a005.
The ability of a series of aromatic and alicyclic analogues of 1,2-ethylenedioxydiacetic acids bearing N,N,N',N'-tetra-n-propyl amide or N-methyl-N-carbethoxypentyl amide linkages to enhance the rate of 45Ca2+ efflux from vesicles was studied. The ligands were less potent in enhancing membrane permeability to Ca2+ than A23187 and X537A. Lipid-soluble anions markedly increased the rate and extent of Ca2+ transport mediated by these neutral ligands. The abilities of these synthetic diamide ligands and naturally occurring ionophores to transport Ca2+ across bilayer membranes were sensitive to the lipid composition of the vesicle. The mechanism of Ca2+ transport mediated by this series of synthetic ligands is discussed.
研究了一系列带有N，N，N'，N'-四正丙基酰胺或N-甲基-N-乙氧羰基戊基酰胺键的1,2-乙二氧基二乙酸的芳香族和脂环族类似物提高45Ca2+从囊泡流出速率的能力。这些配体在增强膜对Ca2+的通透性方面比A23187和X537A效力更低。脂溶性阴离子显著提高了由这些中性配体介导的Ca2+转运的速率和程度。这些合成二酰胺配体和天然存在的离子载体跨双层膜转运Ca2+的能力对囊泡的脂质组成敏感。讨论了由这一系列合成配体介导的Ca2+转运机制。
