Goidl J A, Cake M H, Dolan K P, Parchman L G, Litwack G
Biochemistry. 1977 May 17;16(10):2125-30. doi: 10.1021/bi00629a012.
The rat liver glucorcorticoid receptor has been activated using three procedures: heat, gel filtration, and dilution. With time after heat activation the steroid--receptor complex loses its capacity to bind to DNA--cellulose, while receptor activated by Sephadex G-25 and by dilution maintains DNA--cellulose binding capacity. The rates of steroid dissociation from nonactivated and activated receptor and essentially identical. However, nonactivated receptor is capable of rebinding steroid, while activated receptor has a reduced capacity to rebind steroid. The results of the gel filtration and dilution studies suggest that a low-molecular-weight factor(s) exists in rat liver cytosol which is involved in the process of activation.
加热、凝胶过滤和稀释。加热激活后，随着时间推移，类固醇-受体复合物失去与DNA-纤维素结合的能力，而经葡聚糖凝胶G-25和稀释激活的受体则保持与DNA-纤维素的结合能力。类固醇从未激活和激活的受体上解离的速率基本相同。然而，未激活的受体能够重新结合类固醇，而激活的受体重新结合类固醇的能力降低。凝胶过滤和稀释研究的结果表明，大鼠肝脏细胞质中存在一种低分子量因子，它参与激活过程。
