牛凝血因子XI（血浆凝血活酶前体）的分离与特性研究
Isolation and characterization of bovine factor XI (plasma thromboplastin antecedent).
作者信息
Koide T, Kato H, Davie E W
出版信息
Biochemistry. 1977 May 17;16(10):2279-86. doi: 10.1021/bi00629a037.
Factor XI (plasma thromboplastic antecedent) has been purified approximately 28 000-fold from bovine plasma with an overall yield of about 30%. The isolation procedure involves barium sulfate adsorption of contaminants, ammonium sulfate precipitation, and chromatography on heparin-agarose, CM-Sephadex, and DEAE-Sephadex. The final product was homogeneous when examined by polyacrylamide gel electrophoresis and immunoelectrophoresis. A minimal mol wt of 124 000 was determined by sedimentation equilibrium. Factor XI is composed of two similar or identical polypeptide chain (mol wt of approximately 55 000), and these two chains are held together by a disulfide bond(s). Factor XI is a glycoprotein which contains approximately 11% carbohydrate including 5.4% heose, 4.7% N-acetylhexosamine, and 1.0% N-acetylneuraminic acid. Other properties of this coagulation factor including its amino acid composition and inhibition by antibodies prepared in rabbits are also reported.
凝血因子 XI（血浆凝血活酶前体）已从牛血浆中纯化了约 28000 倍，总收率约为 30%。分离过程包括用硫酸钡吸附杂质、硫酸铵沉淀以及在肝素琼脂糖、CM - 葡聚糖凝胶和 DEAE - 葡聚糖凝胶上进行色谱分离。通过聚丙烯酰胺凝胶电泳和免疫电泳检测时，最终产物是均一的。通过沉降平衡测定其最小分子量为 124000。凝血因子 XI 由两条相似或相同的多肽链（分子量约为 55000）组成，这两条链通过一个或多个二硫键连接在一起。凝血因子 XI 是一种糖蛋白，含有约 11%的碳水化合物，包括 5.4%的己糖、4.7%的 N - 乙酰己糖胺和 1.0%的 N - 乙酰神经氨酸。还报道了这种凝血因子的其他特性，包括其氨基酸组成以及兔制备的抗体对它的抑制作用。
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