Papaphilis A D, Shaw Y H
Biochim Biophys Acta. 1977 May 17;476(2):122-30. doi: 10.1016/0005-2787(77)90089-2.
This study examines certain aspects of the interaction of acridines with DNA. A comparative study of the methods available for the determination of the association constants (Kap) for compounds which interact with DNA has been pursued. A new equation which permits the spectrophotomeric determination of Kap has been derived. This equation can be applied to compounds which upon interaction with a polymer exhibit discrete absorption changes. Application of this equation to substituted acridines and tetrahydroacridines yields some preliminary information on the effect of ring substituents on the interaction of acridines with DNA. Low levels of DNA/dye ratios have been used in the studies reported herein.
本研究考察了吖啶与DNA相互作用的某些方面。对可用于测定与DNA相互作用的化合物的缔合常数（Kap）的现有方法进行了比较研究。推导了一个允许分光光度法测定Kap的新方程。该方程可应用于与聚合物相互作用时表现出离散吸收变化的化合物。将该方程应用于取代吖啶和四氢吖啶，得到了关于环取代基对吖啶与DNA相互作用影响的一些初步信息。本文报道的研究中使用了低水平的DNA/染料比。
