Pace-Asciak C R, Domazet Z, Carrara M
Biochim Biophys Acta. 1977 May 25;487(2):400-4. doi: 10.1016/0005-2760(77)90017-0.
Homogenates of the rat kidney cortex converted 5,8,9,11,12,14,15-hepta-tritiated 6-ketoprostaglandin F 1alpha into one major product identified by gas chromatography-mass spectrometry of the methoxime-methyl ester trimethylsilyl ether derivative as 6,15-diketo-9,11-dihydroxyprost-13-enoic acid. The sequence of derivatisation i.e. methoximation prior to methylation, was crucial as methylation of 15-keto catabolites of the E, F and 6-keto-F series affords degradation products. The corresponding 15-keto-13,14-dihydro catabolite was formed in much smaller quantities. Time course studies indicated that 6-keto-prostaglandin F1alpha was catabolised at a slower rate (about 2-5 fold) than prostaglandin F1alpha. The catabolic activity was blocked by NADH.
大鼠肾皮质匀浆将5,8,9,11,12,14,15-七氚化6-酮前列环素F1α转化为一种主要产物，通过对甲氧肟-甲酯三甲基硅醚衍生物进行气相色谱-质谱分析鉴定为6,15-二酮-9,11-二羟基前列腺-13-烯酸。衍生化顺序，即甲基化之前的甲氧肟化，至关重要，因为E、F和6-酮-F系列的15-酮代谢产物甲基化会产生降解产物。相应的15-酮-13,14-二氢代谢产物生成量要少得多。时间进程研究表明，6-酮前列环素F1α的分解代谢速率比前列腺素F1α慢（约2-5倍）。分解代谢活性被NADH阻断。
