Sajjadi F G, Boyle D L, Domingo R C, Firestein G S
Gensia Inc., San Diego, CA 92121, USA.
FEBS Lett. 1996 Mar 11;382(1-2):125-9. doi: 10.1016/0014-5793(96)00150-0.
A cDNA encoding variant form of the A3 adenosine (Ado) receptor was isolated from rat by reverse transcription of brain mRNA followed by PCR. The full-length receptor (A3i) cDNA encodes 337 amino acids and shares complete sequence identity with the rat A3 Ado receptor, except for the presence of a seventeen amino acid insert located in the second intracellular domain. In contrast to the rat A3 receptor, stable expression of A3i in CHO cells resulted in poor coupling to Gi proteins. Analysis of receptor transcripts by RT-PCR suggests that the A3 Ado receptor mRNAs are products of alternative splicing. Sequence analysis of A3 genomic DNA identified a 1.7 kb intron that is likely alternatively spliced to produce the A3 and A3i receptors.
通过逆转录大鼠脑mRNA并进行PCR，从大鼠中分离出一种编码A3腺苷（Ado）受体变体形式的cDNA。全长受体（A3i）cDNA编码337个氨基酸，与大鼠A3 Ado受体具有完全的序列同一性，但在第二个细胞内结构域存在一个17个氨基酸的插入序列。与大鼠A3受体相反，A3i在CHO细胞中的稳定表达导致与Gi蛋白的偶联较差。通过RT-PCR分析受体转录本表明，A3 Ado受体mRNA是可变剪接的产物。A3基因组DNA的序列分析鉴定出一个1.7 kb的内含子，其可能通过可变剪接产生A3和A3i受体。
