Yamamura S, Sadahira Y, Ruan F, Hakomori S, Igarashi Y
The Biomembrane Institute, Seattle, WA 98119, USA.
FEBS Lett. 1996 Mar 11;382(1-2):193-7. doi: 10.1016/0014-5793(96)00175-5.
Sphingosine-1-phosphate (Sph-1-P), the initial product of sphingosine (Sph) catabolism, has been reported to inhibit motility of mouse melanoma B16/F1 and other types of cells at very low concentrations (10-100 nM). Sph-1-P (100 nM-1 microM) inhibited pseudopodium formation by blocking polymerization and reorganization of actin filaments in newly formed pseudopodia, and reduced F-actin by approximately 25% in F1 cells. A pyrene-labeled actin nucleation assay revealed that Sph-1-P (100 nM) inhibits actin nucleation mediated by F1 cell plasma membranes. These results suggest that Sph-1-P interacts with molecules associated with actin nucleation to inhibit reorganization of pseudopodium formation and cell motility.
鞘氨醇-1-磷酸（Sph-1-P）是鞘氨醇（Sph）分解代谢的初始产物，据报道，它在极低浓度（10-100 nM）下就能抑制小鼠黑色素瘤B16/F1细胞及其他类型细胞的运动。Sph-1-P（100 nM-1 microM）通过阻止新形成伪足中肌动蛋白丝的聚合和重组来抑制伪足形成，并使F1细胞中的F-肌动蛋白减少约25%。芘标记的肌动蛋白成核试验表明，Sph-1-P（100 nM）抑制F1细胞质膜介导的肌动蛋白成核。这些结果表明，Sph-1-P与肌动蛋白成核相关分子相互作用，以抑制伪足形成的重组和细胞运动。
