Pan J, Nakanishi K, Yutsudo M, Inoue H, Li Q, Oka K, Yoshioka N, Hakura A
Department of Tumor Virology, Research Institute for Microbial Diseases, Osaka University, Japan.
FEBS Lett. 1996 Mar 25;383(1-2):21-5. doi: 10.1016/0014-5793(96)00210-4.
We have isolated a novel gene which was expressed in normal rat cells, but completely suppressed in cells transformed by v-src. The molecularly cloned cDNA was about 1.8 kb in size, containing an open reading frame composed of 464 amino acid residues. DNA sequence analysis showed that there was no corresponding gene in the data bases. Besides the suppression of gene expression in the v-src transformed cells, its expression was also strongly suppressed in cells transformed by other oncogenes such as v-abl, v-fps, v-mos, v-sis, v-K-ras, and polyomavirus middle T, but not affected in cells transformed by human papillomavirus type 16 E6E7 and polyomavirus large T. We named the gene drs for a gene down-regulated by v-src.
我们分离出了一个新基因，它在正常大鼠细胞中表达，但在被v-src转化的细胞中完全被抑制。分子克隆的cDNA大小约为1.8 kb，包含一个由464个氨基酸残基组成的开放阅读框。DNA序列分析表明，数据库中没有相应的基因。除了在v-src转化细胞中基因表达受到抑制外，在被其他致癌基因如v-abl、v-fps、v-mos、v-sis、v-K-ras和多瘤病毒中间T转化的细胞中其表达也被强烈抑制，但在被人乳头瘤病毒16型E6E7和多瘤病毒大T转化的细胞中不受影响。我们将该基因命名为drs，即一个被v-src下调的基因。
