酸性磷脂抑制大鼠脑神经元细胞核的磷脂酶D活性。

Acidic phospholipids inhibit the phospholipase D activity of rat brain neuronal nuclei.

作者信息

Kanfer J N, McCartney D G, Singh I N, Freysz L

机构信息

Department of Biochemistry and Molecular Biology, University of Manitoba, Winnipeg, Canada.

出版信息

FEBS Lett. 1996 Mar 25;383(1-2):6-8. doi: 10.1016/0014-5793(96)00205-0.

DOI:10.1016/0014-5793(96)00205-0

PMID:8612792

Abstract

An oleate dependent form of phospholipase D is present in rat brain neuronal nuclei and both the hydrolytic and transphosphatidylation activities measured. Several acidic phospholipids were found to inhibit this activity in a dose dependent manner. The IC50 values varied from 3.5 microM for PIP2 to 200 microM for phosphatidic acid. The hydrolysis of PIP2 by phospholipase C would be expected to result in the disinhibition of the oleate dependent phospholipase D activity.

摘要

大鼠脑神经元细胞核中存在一种油酸依赖性磷脂酶D，并测定了其水解活性和转磷脂酰基活性。发现几种酸性磷脂以剂量依赖性方式抑制该活性。IC50值从磷脂酰肌醇-4,5-二磷酸（PIP2）的3.5微摩尔到磷脂酸的200微摩尔不等。预计磷脂酶C对PIP2的水解会导致油酸依赖性磷脂酶D活性的去抑制。