Exner T, Rickard K A
Biomedicine. 1977 Mar;27(2):62-5.
The activation of prothrombin concentrate mixtures incubated "in vitro" was investigated. "Activation" was assessed by the correcting effect of suct mixtures on the APTT of a test plasma containing a factor VIII inhibitor and also by the generation of free thrombin. Thrombin itself was found to have insignificant activating effect. Prothrombin concentrates incubated with near-physiological levels of calcium appeared to correct the abnormal APTT to an increasing degree as the calcium concentration was increased. Free thrombin was generated with calcium cencentrations greater than those required for APTT correction. The activation process could be stopped at any stage by adding citrate which removed ionized calcium from the incubation mixture.
对在“体外”孵育的凝血酶原浓缩物混合物的激活情况进行了研究。通过这些混合物对含有因子VIII抑制剂的测试血浆的活化部分凝血活酶时间（APTT）的校正作用以及游离凝血酶的生成来评估“激活”情况。发现凝血酶本身的激活作用不显著。与接近生理水平的钙一起孵育的凝血酶原浓缩物，随着钙浓度的增加，似乎能越来越大地校正异常的APTT。当钙浓度高于校正APTT所需的浓度时会生成游离凝血酶。通过加入柠檬酸盐从孵育混合物中去除离子钙，可在任何阶段停止激活过程。
