Teixeira P, Menezes S
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Int J Radiat Biol. 1996 Mar;69(3):345-50. doi: 10.1080/095530096145904.
Methylene blue (MB) is shown to sensitize E. coli cells to the effects of X-rays. This sensitization is dependent on factors such as dye concentration, incubation temperature, membrane permeability, and repair capacities, suggesting that the binding and/or penetration of the dye into the cells determines the potentiation of the lethal effects of X-rays by MB. It is also demonstrated that the presence of the polymerase 1 enzyme is essential for this sensitization to take place. Since MB is known to penetrate, accumulate, and be retained preferentially in some malignant tissues, the possibility of using this dye as a specific sensitizer to X-rays in the radiotherapy of some cancers is discussed.
亚甲蓝（MB）已被证明可使大肠杆菌细胞对X射线的作用敏感。这种敏感性取决于染料浓度、孵育温度、膜通透性和修复能力等因素，这表明染料与细胞的结合和/或渗透决定了MB对X射线致死效应的增强作用。还证明了聚合酶1酶的存在对于这种敏化作用的发生至关重要。由于已知MB可优先穿透、积累并保留在某些恶性组织中，因此讨论了在某些癌症的放射治疗中使用这种染料作为X射线特异性敏化剂的可能性。
