Meloni-Bruneri L H, Campa A, Abdalla D S, Calich V L, Lenzi H L, Burger E
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brasil.
J Leukoc Biol. 1996 Apr;59(4):526-33. doi: 10.1002/jlb.59.4.526.
The oxidative burst of polymorphonuclear neutrophils (PMN) and their ability to inhibit Paracoccidioides brasiliensis growth was studied in susceptible (B10.A) and resistant (A/J) mice. The cells were obtained after subcutaneous inoculation in air pouches, yielding highly pure PMN preparations; the number of cells was similar for both strains at 24 h and five times higher in the resistant strain at 15 days. The oxidative metabolism of these PMN was evaluated by the luminol and lucigen-enhanced chemiluminescence upon stimulation with PMA or killed P. brasiliensis (Pb). At 24 h of infection PMN from both strains showed similar responses. However, at 15 days a great enhancement of the Pb-stimulated luminol-enhanced chemiluminescence was observed only in PMN from resistant mice. Such increase was markedly inhibited by the addition of catalase. Independent of the mouse strain or time of infection of lucigen-enhanced chemiluminescence showed the same intensity. The lucigen-enhanced chemiluminescence of PMN without stimuli from resistant mice did not change with the time of infection, however, after 15 days of infection a significantly lower chemiluminescence was detected with PMN from susceptible mice. At 15 days of infection the PMN from B10.A were unable to kill P. brasiliensis yeast cells in vitro. Because the lucigenin- and luminol-enhanced chemiluminescence detects, respectively, the O2- production and the myeloperoxidase/hydrogen peroxide halide system, the present data show parallels between deficiency in the production of oxygen-reactive species by PMN and lower fungicidal activity.
在易感（B10.A）和抗性（A/J）小鼠中研究了多形核中性粒细胞（PMN）的氧化爆发及其抑制巴西副球孢子菌生长的能力。细胞是在气袋中皮下接种后获得的，得到了高度纯化的PMN制剂；在24小时时，两种品系的细胞数量相似，而在15天时，抗性品系的细胞数量高出五倍。通过鲁米诺和光泽精增强的化学发光法，在用佛波酯（PMA）或灭活的巴西副球孢子菌（Pb）刺激后，评估这些PMN的氧化代谢。在感染24小时时，两个品系的PMN表现出相似的反应。然而，在15天时，仅在抗性小鼠的PMN中观察到Pb刺激的鲁米诺增强化学发光有很大增强。加入过氧化氢酶可显著抑制这种增加。与小鼠品系或感染时间无关，光泽精增强的化学发光显示相同的强度。来自抗性小鼠的未受刺激的PMN的光泽精增强化学发光不会随感染时间而变化，然而，在感染15天后，易感小鼠的PMN检测到的化学发光明显较低。在感染15天时，来自B10.A的PMN在体外无法杀死巴西副球孢子菌酵母细胞。由于光泽精和鲁米诺增强的化学发光分别检测超氧阴离子（O2-）的产生和髓过氧化物酶/过氧化氢卤化物系统，目前的数据表明PMN中氧活性物质产生的缺陷与较低的杀真菌活性之间存在平行关系。
