Head R J, Stitzel R E, de la Lande I S, Johnson S M
Blood Vessels. 1977;14(4):229-39. doi: 10.1159/000158131.
When assayed on homogenates of the rabbit ear artery up to 192 h after sympathetic denervation, the activity of monoamine oxidase (MAO) showed a small but significant decrease (maximum 9%). The activity of catechol-O-methyl transferase (COMT) appeared unchanged, although it is possible that the small and variable nature of this enzyme activity compared with that of MAO may have masked a comparable effect of denervation. The maximum decreases in noradrenaline (NA) and adenosine triphosphate (ATP) contents were greater than 90% at 48 h, and 32% at 96 h after denervation. These results confirm the presence of neuronal as well as extra-neuronal MAO, and also the presence of extraneuronal comt in this artery. The decline in ATP contents after denervation suggest that part of the ATP is neuronal. However, the estimated ratio of NA to neuronal ATP is far in excess of that reported for isolated noradrenaline storage vesicles, suggesting that the major portion of the neuronal ATP was not present in these vesicles.
在对去交感神经后长达192小时的兔耳动脉匀浆进行检测时，单胺氧化酶（MAO）的活性呈现出虽小但显著的下降（最大降幅为9%）。儿茶酚-O-甲基转移酶（COMT）的活性似乎未发生变化，不过与MAO相比，该酶活性小且变化不定，这可能掩盖了去神经支配的类似效应。去神经支配后48小时，去甲肾上腺素（NA）和三磷酸腺苷（ATP）含量的最大降幅超过90%，96小时时为32%。这些结果证实了该动脉中存在神经元型以及非神经元型MAO，同时也存在非神经元型COMT。去神经支配后ATP含量的下降表明部分ATP是神经元性的。然而，NA与神经元ATP的估计比值远高于分离的去甲肾上腺素储存囊泡所报告的比值，这表明神经元ATP的主要部分并不存在于这些囊泡中。
