从古代组织中进行DNA损伤分析及DNA序列检索。
DNA damage and DNA sequence retrieval from ancient tissues.
作者信息
Höss M, Jaruga P, Zastawny T H, Dizdaroglu M, Pääbo S
机构信息
Zoological Institute, University of Munich, Germany.
出版信息
Nucleic Acids Res. 1996 Apr 1;24(7):1304-7. doi: 10.1093/nar/24.7.1304.
Abstract
Gas chromatography/mass spectrometry (GC/MS) was used to determine the amounts of eight oxidative base modifications in DNA extracted from 11 specimens of bones and soft tissues, ranging in age from 40 to >50 000 years. Among the compounds assayed hydantoin derivatives of pyrimidines were quantitatively dominant. From five of the specimens endogenous ancient DNA sequences could be amplified by PCR. The DNA from these specimens contained substantially lower amounts of hydantoins than the six specimens from which no DNA could be amplified. Other types of damage, e.g. oxidation products of purines, did not correlate with the inability to retrieve DNA sequences. Furthermore, all samples with low amounts of damage and from which DNA could be amplified stemmed from regions where low temperatures have prevailed throughout the burial period of the specimens.
摘要
采用气相色谱/质谱联用技术（GC/MS）测定了从11份骨骼和软组织标本中提取的DNA的8种氧化碱基修饰产物的含量，这些标本的年代跨度为40年至5万多年。在所检测的化合物中，嘧啶的乙内酰脲衍生物在数量上占主导地位。从其中5份标本中可通过聚合酶链反应（PCR）扩增出内源性古代DNA序列。这些标本的DNA中乙内酰脲的含量明显低于另外6份无法扩增出DNA的标本。其他类型的损伤，如嘌呤的氧化产物，与无法获取DNA序列并无关联。此外，所有损伤程度低且能扩增出DNA的样本均来自标本埋藏期间一直处于低温状态的地区。
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