Handt O, Höss M, Krings M, Pääbo S
Zoological Institute, University of Munich, Germany.
Experientia. 1994 Jun 15;50(6):524-9. doi: 10.1007/BF01921720.
The study of ancient DNA offers the possibility of following genetic change over time. However, the field is plagued by a problem which is unique in molecular biology--the difficulty of verifying results by reproduction. Some of the reasons for this are technical and derive from the low copy number and damaged state of ancient DNA molecules. Other reasons are the unique nature of many of the objects from which DNA is extracted. We describe methodological approaches with which these problems can be alleviated in order to ensure that results are scientific in the sense that they can be reproduced by others.
对古DNA的研究为追踪基因随时间的变化提供了可能性。然而，该领域受到分子生物学中一个独特问题的困扰——难以通过重复实验来验证结果。造成这种情况的一些原因是技术性的，源于古DNA分子的低拷贝数和受损状态。其他原因则是许多用于提取DNA的样本具有独特性质。我们描述了一些方法，通过这些方法可以缓解这些问题，以确保结果具有科学性，即其他人能够重复得出这些结果。
