完全编辑和部分编辑的nad9转录本在大小上有所不同，并且二者都与马铃薯线粒体中的多核糖体相关联。
Fully edited and partially edited nad9 transcripts differ in size and both are associated with polysomes in potato mitochondria.
作者信息
Lu B, Hanson M R
机构信息
Department of Genetics and Development, Cornell University, Ithaca, NY 14853, USA.
出版信息
Nucleic Acids Res. 1996 Apr 1;24(7):1369-74. doi: 10.1093/nar/24.7.1369.
Abstract
Two classes of nad9 transcripts are present at different abundances in the steady-state RNA pool of potato mitochondria. The 5'- and 3' termini of the transcripts were determined by primer extension and S1 nuclease protection analyses respectively. Using two primer pairs that will either specifically amplify the larger transcript or amplify both the larger and the smaller transcripts in RT-PCR analyses it was found that the larger nad9 transcripts are partially edited, while the smaller transcripts are fully edited. Both the larger and the smaller transcripts were found to be associated with mitochondrial polysomes. The polysome association was found to be sensitive to EDTA and puromycin treatment. Therefore, both fully and partially edited nad9 transcripts appear to be engaged in translation.
摘要
在马铃薯线粒体的稳态RNA库中，存在两类丰度不同的nad9转录本。转录本的5'端和3'端分别通过引物延伸和S1核酸酶保护分析来确定。在逆转录聚合酶链反应（RT-PCR）分析中，使用两对引物，一对引物特异性扩增较大的转录本，另一对引物同时扩增较大和较小的转录本，结果发现较大的nad9转录本部分被编辑，而较小的转录本则完全被编辑。研究发现，较大和较小的转录本都与线粒体多核糖体相关联。多核糖体的关联对乙二胺四乙酸（EDTA）和嘌呤霉素处理敏感。因此，完全编辑和部分编辑的nad9转录本似乎都参与了翻译过程。
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