微柱中转录的动力学
Kinetics of transcription in a minute column.
作者信息
Kubori T, Shimamoto N
机构信息
DNA Research Center, National Institute of Genetics, The Graduate University for Advanced Studies, Mishima, Japan.
出版信息
Nucleic Acids Res. 1996 Apr 1;24(7):1380-1. doi: 10.1093/nar/24.7.1380.
Abstract
Immobilized enzymes or nucleic acids are widely used to analyze association and dissociation reactions. A pseudo-rapid kinetic method for studying transcription was developed by using an immobilized template DNA packed in a minute column, in a micro-scale liquid chromatography system. This method, which has a dead-time of only several seconds, is quick enough to allow analysis of the release of product RNA in transcription by Escherichia coli RNA polymerase. The method could be applied to most enzymes that interact with DNA.
摘要
固定化酶或核酸被广泛用于分析缔合和解离反应。通过在微尺度液相色谱系统中使用填充在微小柱中的固定化模板DNA，开发了一种研究转录的伪快速动力学方法。这种方法的死时间仅为几秒，足够快以允许分析大肠杆菌RNA聚合酶转录过程中产物RNA的释放。该方法可应用于大多数与DNA相互作用的酶。
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