Ré R N, Cook J L
Alton Ochsner Medical Foundation, Division of Research, New Orleans, Louisiana, USA.
Am J Med Sci. 1996 Feb;311(2):65-72. doi: 10.1097/00000441-199602000-00002.
A number of oligonucleotides were designed to bind through Hoogsteen triple helix or Watson-Crick hydrogen bonds to the p53 consensus sequence homology localized within the human nontranscribed rRNA spacer region. The oligomers, which bind in vitro to the consensus sequence homology, function as p53 analogues in cells deficient in wild-type p53 protein. Oligomers suppress proliferation of human colon cancer cells by three to eightfold, but only suppress proliferation of normal human mesangial cells or lung fibroblasts by less than 50%. On the basis of these studies, p53 analogues may be used therapeutically to selectively modify proliferation of transformed cells.
设计了多种寡核苷酸，使其通过Hoogsteen三链螺旋或沃森-克里克氢键与位于人类非转录rRNA间隔区的p53共有序列同源性区域结合。这些在体外与共有序列同源性结合的寡聚物，在缺乏野生型p53蛋白的细胞中发挥p53类似物的作用。寡聚物可将人类结肠癌细胞的增殖抑制三至八倍，但对正常人系膜细胞或肺成纤维细胞增殖的抑制率不到50%。基于这些研究，p53类似物可用于治疗，以选择性地改变转化细胞的增殖。
