Chew E C, Wu S, Yam H F, Yang L, Cheng-Chew S B, Zheng J, Wu B Q
Department of Anatomy, Chinese University of Hong Kong, Shatin, N.T.
Anticancer Res. 1996 Jan-Feb;16(1):353-7.
Many potentially useful antigens have been difficult to detect in formalin-fixed, paraffin-embedded tissues. Recently a number of pathological and research laboratories have demonstrated that some antigens masked by formalin fixation could be restored to detectability by microwave heating. Previously, we were unable to demonstrate laminin receptor in cells processed by the routine fixation. Our results showed that microwave heating together with trypsin produced the best immunohistochemical staining for this receptor. Nevertheless, no significance was found in the levels of 67 kD LR in high and low metastatic tumor cell lines.
许多潜在有用的抗原在福尔马林固定、石蜡包埋的组织中难以检测到。最近，一些病理和研究实验室表明，一些被福尔马林固定所掩盖的抗原可以通过微波加热恢复可检测性。此前，我们无法在常规固定处理的细胞中检测到层粘连蛋白受体。我们的结果表明，微波加热结合胰蛋白酶对该受体产生了最佳的免疫组织化学染色。然而，在高转移和低转移肿瘤细胞系中，67 kD层粘连蛋白受体水平未发现显著差异。
