Sallot M, Ordener C, Lascombe I, Propper A, Adessi G L, Jouvenot M
Service de Biochimie-Biologie, Moléculaire, Institut d'Etude et de Transfert de Gènes, Besancon, France.
Anticancer Res. 1996 Jan-Feb;16(1):401-6.
Our aim was to analyze EGF action on nuclear protooncogenes in RL95-2 since it has not been documented so far. Synchronization and partial' growth arrest were obtained by maintaining cells for 15 hours in L-methionine-free medium. After this depletion, EGF transiently increased fos and jun mRNAs: the expression peaked at 45 minutes for c-fos (5.5 fold induction) and at 60 minutes for c-jun and jun-B (3 fold induction) and the mRNA levels returned to the basal value within 3 hours. Upon EGF addition, c-myc mRNAs peaked at 12 hours (7.6 fold induction) and surprisingly remained higher than the control up to 48 hours. Unlike fetal calf serum, EGF did not increase the cell number and this could be linked to steadily induced c-myc expression. These data provide evidence for a differential EGF action on fos/jun and c-myc in RL95-2 cells.
我们的目的是分析表皮生长因子（EGF）对RL95-2细胞核原癌基因的作用，因为迄今为止尚无相关文献记载。通过在无L-甲硫氨酸的培养基中培养细胞15小时来实现同步化和部分生长停滞。在此耗尽后，EGF短暂增加了fos和jun mRNA：c-fos的表达在45分钟时达到峰值（诱导5.5倍），c-jun和jun-B在60分钟时达到峰值（诱导3倍），且mRNA水平在3小时内恢复到基础值。添加EGF后，c-myc mRNA在12小时时达到峰值（诱导7.6倍），令人惊讶的是，直至48小时仍高于对照。与胎牛血清不同，EGF并未增加细胞数量，这可能与持续诱导的c-myc表达有关。这些数据为EGF对RL95-2细胞中fos/jun和c-myc的不同作用提供了证据。
