Hayashi M, Tsugeki R, Kondo M, Mori H, Nishimura M
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
Plant Mol Biol. 1996 Jan;30(1):183-9. doi: 10.1007/BF00017813.
Two full-length cDNAs encoding hydroxypyruvate reductase were isolated from a cDNA library constructed with poly(A)+ RNA from pumpkin green cotyledons. One of the cDNAs, designated HPR1, encodes a polypeptide of 386 amino acids, while the other cDNA, HPR2 encodes a polypeptide of 381 amino acids. Although the nucleotide and deduced amino acid sequences of these cDNAs are almost identical, the deduced HPR1 protein contains Ser-Lys-Leu at its carboxy-terminal end, which is known as a microbody-targeting signal, while the deduced HPR2 protein does not. Analysis of genomic DNA strongly suggests that HPR1 and HPR2 are produced by alternative splicing.
从用南瓜绿色子叶的聚腺苷酸加尾RNA构建的cDNA文库中分离出两个编码羟基丙酮酸还原酶的全长cDNA。其中一个cDNA命名为HPR1，编码一个386个氨基酸的多肽，而另一个cDNA，HPR2编码一个381个氨基酸的多肽。尽管这些cDNA的核苷酸和推导的氨基酸序列几乎相同，但推导的HPR1蛋白在其羧基末端含有丝氨酸-赖氨酸-亮氨酸，这是一种已知的微体靶向信号，而推导的HPR2蛋白则没有。基因组DNA分析强烈表明，HPR1和HPR2是通过可变剪接产生的。
