Baratti C M, Kopf S R
Laboratorio de Neurofarmacologia de Procesos de Memoria, Catedra de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 9560, Buenos Aires, RA-1113, Argentina.
Neurobiol Learn Mem. 1996 May;65(3):197-201. doi: 10.1006/nlme.1996.0023.
Posttraining administration of the L-enantiomer of the competitive inhibitor of nitric oxide synthase, NG-nitro-L-arginine methyl ester (L-NAME, 3-100 mg/kg, ip), impaired 48-h retention of a one-trial step-through inhibitory shock-avoidance task in male Swiss mice. The effects were dose-dependent and were not observed when the D-enantiomer (D-NAME, 3-100 mg/kg, ip) was injected instead of L-NAME. Retention latencies of mice that had not received a footshock during training were not affected by L-NAME. The memory impairment produced by L-NAME was time-dependent, suggesting an action on memory storage. The effects of L-NAME on memory were overcome by the injection of L-(but not D-)arginine (300 mg/kg, ip) along with the inhibitor. Considered together, these findings suggest that the L-arginine/nitric oxide pathway may be involved in memory storage of an inhibitory avoidance response in mice.
一氧化氮合酶竞争性抑制剂L-硝基精氨酸甲酯（L-NAME，3-100毫克/千克，腹腔注射）在训练后给药，会损害雄性瑞士小鼠对一次性穿梭抑制性休克回避任务的48小时记忆保持。这些效应具有剂量依赖性，而当注射D-对映体（D-NAME，3-100毫克/千克，腹腔注射）而非L-NAME时则未观察到这种效应。训练期间未接受足部电击的小鼠的记忆保持潜伏期不受L-NAME影响。L-NAME产生的记忆损害具有时间依赖性，表明其对记忆存储有作用。L-精氨酸（而非D-精氨酸，300毫克/千克，腹腔注射）与抑制剂一起注射可克服L-NAME对记忆的影响。综合来看，这些发现表明L-精氨酸/一氧化氮途径可能参与小鼠抑制性回避反应的记忆存储。
