Leishmania chagasi: a gene encoding a protein kinase with a catalytic domain structurally related to MAP kinase kinase.

The gene for a serine/threonine protein kinase was isolated from Leishmania chagasi. The deduced amino acid sequence encoded by this Leishmania protein kinase (LPK-1) gene possesses all of the 11 conserved subdomains found in most other serine/threonine protein kinase catalytic domains. Sequence alignment with other known protein kinases demonstrates that the 42-kDa LPK-1 is a member of the mitogen-activated protein kinase kinase family involved in the signal-transduction pathways in yeast and mammalian cells. The single copy gene for LPK-1 specifies a 3.1-kb mRNA that is expressed in both logarithmic and stationary phase promastigotes with a twofold higher steady-state level in the infective stationary phase promastigotes.