用抗胼胝体抗血清检测到的脑细胞表面抗原。

Brain cell surface antigens detected by anti-corpus callosum antiserum.

作者信息

Schachner M, Wortham K A, Ruberg M Z, Dorfman S, Campbell G L

出版信息

Brain Res. 1977 May 20;127(1):87-97. doi: 10.1016/0006-8993(77)90381-x.

Abstract

When rabbits are injected with tissue homogenates of white matter from bovine corpus callosum, an antiserum is produced which reacts with the surface membrane of 20-30% of all cells obtained by trypsin-dissociation of cerebellum from 10-day-old mice. The antigen or set of antigens recognized by this antiserum is detectable on embryonic, early postnatal, and adult mouse brain, but not on liver, spleen, kidney, thymus and sperm. The antigen is expressed in different regions of the brain and also, in decreased amounts, on retina. In histological sections of cerebellum from 21-day-old mice the antigen is predominantly localized in white matter tracts. Whereas nervous tissue from chicken and rabbit does not carry detectable levels of the antigen, rat, bovine and human brains are antigen-positive.

摘要

当给兔子注射来自牛胼胝体白质的组织匀浆时，会产生一种抗血清，该抗血清能与通过胰蛋白酶解离10日龄小鼠小脑获得的所有细胞中20%-30%的细胞膜发生反应。这种抗血清识别的抗原或一组抗原在胚胎期、出生后早期和成年小鼠大脑中均可检测到，但在肝脏、脾脏、肾脏、胸腺和精子中则检测不到。该抗原在大脑的不同区域表达，在视网膜上的表达量也有所减少。在21日龄小鼠小脑的组织切片中，该抗原主要定位于白质束。而鸡和兔子的神经组织中未检测到可检测水平的该抗原，大鼠、牛和人类大脑则为抗原阳性。