Tong S Y, Hutson J M, Watts L M
Surgical Research Laboratory, Royal Children's Hospital Research Foundation, Melbourne, Australia.
J Urol. 1996 Jun;155(6):2057-9.
Sexual differentiation in gonadal dysgenesis is commonly asymmetrical. In patients with true hermaphroditism there may be an ovary and müllerian duct on 1 side, and a testis and wolffian duct on the other side. Such asymmetry suggests that testicular hormones only act locally at this early stage of sexual differentiation. We tested the hypothesis that testosterone reaches the wolffian duct by transport down the duct rather than by simple diffusion.
Mouse 14-day urogenital ridges were placed in organ culture and microinjected with testosterone-albumin-fluorescein isothiocyanate.
At 17 hours fluorescence was found throughout the wolffian duct and by 48 hours it was maximal in the dilated caudal end.
Our results support the hypothesis that androgens may be transported along the wolffian duct. Secretion of testicular hormones into the wolffian duct may maintain hormone levels in the biologically active range.
性腺发育不全中的性分化通常是不对称的。在真两性畸形患者中，一侧可能有卵巢和苗勒管，另一侧有睾丸和沃尔夫管。这种不对称表明，睾丸激素在性分化的早期仅在局部起作用。我们检验了这样一个假说，即睾酮是通过沿着管道运输而非简单扩散到达沃尔夫管的。
将14日龄小鼠的泌尿生殖嵴置于器官培养中，并显微注射睾酮-白蛋白-异硫氰酸荧光素。
17小时时，在整个沃尔夫管中发现荧光，48小时时，在扩张的尾端荧光最强。
我们的结果支持雄激素可能沿沃尔夫管运输的假说。睾丸激素分泌到沃尔夫管中可能使激素水平维持在生物活性范围内。
