Orlov E N, Antipov E M
Med Tekh. 1995 Sep-Oct(5):38-9.
The paper describes a simple way of updating a Russian production-type LKaM-80 chromatograph in order to make a gas chromatographic analysis of urinary steroids for separation by using high performance capillary columns. To connect capillary columns, the units consisting of a gas heaving T-joint, a stream separating chamber, and a releasing restrictor were applied. To achieve satisfactory results, it is necessary to place a column through the tube connecting the union for attachment of the column and the flame-ionization detector so that the end of a capillary should be located at the detector as soon as closer. The updated chromatograph has been successfully used to obtain urinary steroidal profiles which are essential for the differential diagnosis of causes of hyperandrogyny in females, including pregnants.
本文描述了一种更新俄罗斯生产型LKaM - 80色谱仪的简单方法，以便使用高效毛细管柱对尿甾体进行气相色谱分析分离。为连接毛细管柱，采用了由气体提升T形接头、气流分离室和释放限流器组成的单元。为获得满意结果，需将色谱柱穿过连接柱附件接头与火焰离子化检测器的管子，使毛细管末端尽可能靠近检测器。更新后的色谱仪已成功用于获取尿甾体谱，这对于女性包括孕妇雄激素过多病因的鉴别诊断至关重要。
