Magnusson P, Häger A, Larsson L
Department of Clinical Chemistry, Linköping University Hospital, Sweden.
Pediatr Res. 1995 Dec;38(6):955-61. doi: 10.1203/00006450-199512000-00021.
We report reference values for osteocalcin and bone and liver alkaline phosphatase (ALP) isoforms in serum from 114 healthy children and adolescents, age 7-16 y. The bone and liver ALP isoforms were measured by high performance liquid chromatography (HPLC). Six ALP isoforms were separated and quantified in all samples: one bone/intestinal (B/I), two bone (B1 and B2), and three liver ALP isoforms (L1, L2, and L3). It is especially important to make distinctions between ages and gender during puberty in the interpretation of biochemical markers of bone. We observed that girls reached adult levels for osteocalcin, total ALP, and bone ALP isoforms, B/I, B1, and B2, earlier than boys. Girls in the oldest age group (15-16 y) and in the pubertal stage groups IV-V, reached higher levels than boys for the calculated ratio B1/B2 due to a more rapid decline of B2 compared with B1. This observation might indicate that B2, to a higher extent than the B1 isoform, represent a more mature expression of osteoblast differentiation during matrix mineralization in growing bone. Serial measurements of these new bone ALP isoforms could provide new insights into bone and mineral metabolism.
我们报告了114名7至16岁健康儿童和青少年血清中骨钙素、骨和肝碱性磷酸酶（ALP）同工酶的参考值。骨和肝ALP同工酶通过高效液相色谱法（HPLC）测定。在所有样本中分离并定量了六种ALP同工酶：一种骨/肠型（B/I）、两种骨型（B1和B2）以及三种肝ALP同工酶（L1、L2和L3）。在解释骨生化标志物时，区分青春期的年龄和性别尤为重要。我们观察到，女孩的骨钙素、总ALP以及骨ALP同工酶B/I、B1和B2达到成人水平的时间比男孩早。在最大年龄组（15 - 16岁）以及青春期IV - V阶段组的女孩中，由于B2与B1相比下降更快，计算得出的B1/B2比值高于男孩。这一观察结果可能表明，与B1同工酶相比，B2在更大程度上代表了生长骨基质矿化过程中成骨细胞分化的更成熟表达。对这些新的骨ALP同工酶进行连续测量可能会为骨和矿物质代谢提供新的见解。
