Ho P P, Couch F J, Brody L C, Abel K J, Boehnke M, Shearon T H, Chandrasekharappa S C, Collins F S, Weber B L
Wayne State University, Department of Immunology and Microbiology, Detroit, Michigan 48201, USA.
Somat Cell Mol Genet. 1995 Sep;21(5):351-5. doi: 10.1007/BF02257470.
The human homolog of the Saccharomyces cerevisiae cell division control 27 gene (CDC27) was mapped to human chromosome 17q12-q21 using a panel of human/rodent somatic cell hybrids and localized distal to the breast cancer susceptibility gene, BRCA1, using a panel of radiation hybrids. The radiation hybrid panel indicates that the most likely position of human CDC27 on human chromosomes 17 is between the marker D17S409 and the beta 3 subunit of integrin (ITGB3). Further confirmation of this localization comes from the sequence tagged site (STS) mapping of human CDC27 to the same yeast artificial chromosomes (YACs) positive for ITGB3. The estimated distance between ITGB3 and human CDC27 is less than 600 kb.
利用一组人/啮齿动物体细胞杂种，将酿酒酵母细胞分裂控制27基因（CDC27）的人类同源基因定位到人类染色体17q12 - q21；利用一组辐射杂种，将其定位到乳腺癌易感基因BRCA1的远端。辐射杂种面板表明，人类CDC27在人类染色体17上最可能的位置在标记D17S409和整合素β3亚基（ITGB3）之间。人类CDC27的序列标签位点（STS）定位到与ITGB3呈阳性的相同酵母人工染色体（YAC）上，进一步证实了这一定位。ITGB3与人类CDC27之间的估计距离小于600 kb。
