The fully automatic ion-exchange and gel-permeation chromatography of neutral monosaccharides and oligosaccharides with a Jeolco JLc-6AH analyser.

Experiences with a Jeolco JLC-6AH carbohydrate analyser used to provide a fully automated analysis service for neutral mono- and oligo-saccharides have been summarised. Modifications have been made to the equipment to extend its application, and a method for routine separations of oligosaccharides (d.p. 2--15) has been established. The degree of separation achieved is far superior to that which may be obtained by gel-filtration techniques under gravity- or peristaltic-pump-aided flow. The versatility and adaptability of equipment for use with assays other than the standard orcinol-sulphuric acid reaction shows that it is not too inconvenient to interchange between various assays and separations.