Knöll R, Zimmermann R, Arras M, Schaper W
Department of Experimental Cardiology, Max-Planck-Institut, Bad Nauheim, Germany.
Biochem Biophys Res Commun. 1996 Apr 16;221(2):402-7. doi: 10.1006/bbrc.1996.0608.
For the study of ischemia-related adaptations we employed DDRT-PCR on mRNA obtained from heart regions undergoing brief (10') occlusions/reperfusions. We found and sequenced 52 differentially expressed clones that we further characterized using Northern analysis with RNA from a wide variety of tissues. We selected only clones (1) with a new sequence, (2) that showed a Northern signal, and (3) that exhibited organ-specific (heart, muscle) or (4) situation-specific (ischemia, non-specific stress) expression. We found two new heart-specific transcripts and three new stress-inducible genes. The transcripts with known sequences showed an expression pattern typical for repair processes after ischemia-reperfusion (ubiquitin, ATPases). About 20% of clones were truly differentially expressed.
为了研究缺血相关适应性，我们对从经历短暂（10分钟）闭塞/再灌注的心脏区域获得的mRNA进行了DDRT-PCR。我们发现并测序了52个差异表达的克隆，并用来自多种组织的RNA通过Northern分析进一步对其进行了表征。我们仅选择了以下克隆：（1）具有新序列的；（2）显示Northern信号的；（3）表现出器官特异性（心脏、肌肉）或（4）情况特异性（缺血、非特异性应激）表达的。我们发现了两个新的心脏特异性转录本和三个新的应激诱导基因。具有已知序列的转录本显示出缺血再灌注后修复过程的典型表达模式（泛素、ATP酶）。约20%的克隆是真正差异表达的。
