Mesquita R, Björkholm M, Ekman M, Bogdanovic G, Biberfeld P
Immunopathology Laboratory, Department of Pathology, Karolinska Institute/Hospital, Stockholm, Sweden.
APMIS. 1996 Feb;104(2):153-60. doi: 10.1111/j.1699-0463.1996.tb00701.x.
Polyomavirus-infected oligodendrocytes and in some instances virus-carrying macrophages were demonstrated within the cytoplasm of astrocytes by double immunostaining of brain lesions in a case of PML following bone marrow transplantation. The infected oligodendrocytes and CD68+ cells were usually partially or completely invested by the cytoplasm of reactive and infected or noninfected giant pleomorphic astrocytes. By electron microscopy (EM), internalized infected oligodendrocytes were shown to release polyomavirus particles possibly infectious to the astrocytes. These observations seem to indicate that astrocytes in PML can become infected by direct contact with internalized infected oligodendrocytes and possibly also by virus-loaded macrophages. Correspondingly, astrocyte/oligodendrocyte/macrophage cell-to-cell interactions appear to play an important role in the pathogenesis of PML lesions.
在一例骨髓移植后发生进行性多灶性白质脑病（PML）的病例中，通过对脑损伤进行双重免疫染色，在星形胶质细胞的细胞质内发现了多瘤病毒感染的少突胶质细胞，在某些情况下还发现了携带病毒的巨噬细胞。受感染的少突胶质细胞和CD68 +细胞通常被反应性的、受感染或未受感染的巨大多形性星形胶质细胞的细胞质部分或完全包裹。通过电子显微镜（EM）观察发现，内化的受感染少突胶质细胞会释放可能感染星形胶质细胞的多瘤病毒颗粒。这些观察结果似乎表明，PML中的星形胶质细胞可通过与内化的受感染少突胶质细胞直接接触而被感染，也可能通过携带病毒的巨噬细胞感染。相应地，星形胶质细胞/少突胶质细胞/巨噬细胞之间的细胞间相互作用似乎在PML病变的发病机制中起重要作用。
