Roy S, Sedqi M, Ramakrishnan S, Barke R A, Loh H H
Department of Surgery, University of Minnesota, Minneapolis 55455, USA.
Cell Immunol. 1996 May 1;169(2):271-7. doi: 10.1006/cimm.1996.0118.
Chronic treatment of mice with morphine selectively abrogates the terminal differentiation of committed bone marrow progenitor cells to form macrophage colony forming units. To understand the molecular mechanisms involved in morphine-mediated suppression of myeloid cell differentiation, we investigated the use of a macrophage cell line, Bac 1.2 F5. In vitro proliferation of this cell line is dependent on the exogenous supply of macrophage colony stimulating factor. Treatment of Bac 1.2F5 cells in vitro with morphine showed a dose-dependent inhibition of proliferation which was associated with morphological changes. Characterization of the binding site revealed that the binding site for morphine on these cells is different from the classical opioid receptors described in the brain. In addition to the putative novel class of morphine receptors, Bac 1.2F5 cells also expressed the delta opioid receptors as determined by RT-PCR analyses. These studies show that Bac 1.2F5 cells are suitable for the molecular characterization of opioid effects on the proliferation and differentiation of myeloid progenitor cells.
用吗啡对小鼠进行长期治疗可选择性地消除定向骨髓祖细胞的终末分化，从而形成巨噬细胞集落形成单位。为了了解吗啡介导的骨髓细胞分化抑制所涉及的分子机制，我们研究了巨噬细胞系Bac 1.2 F5的使用情况。该细胞系的体外增殖依赖于巨噬细胞集落刺激因子的外源供应。在体外使用吗啡处理Bac 1.2F5细胞显示出剂量依赖性的增殖抑制，这与形态学变化有关。结合位点的表征显示，这些细胞上吗啡的结合位点与大脑中描述的经典阿片受体不同。除了推测的新型吗啡受体类别外，通过RT-PCR分析确定，Bac 1.2F5细胞还表达δ阿片受体。这些研究表明，Bac 1.2F5细胞适用于对阿片类药物对骨髓祖细胞增殖和分化影响的分子表征。
