一种直接测定生物流体中总二氧化碳含量的简单微量方法。

A simple micromethod for the direct determination of the total carbon dioxide content in biological fluids.

作者信息

Van Stekelenburg G J, De Keyzer M H, Donckerwolcke R A, Maas A H, Van De Camp R A

出版信息

Clin Chim Acta. 1977 Apr 15;76(2):161-9. doi: 10.1016/0009-8981(77)90091-2.

Abstract

A simple method is presented for the determination of the total carbon dioxide content applicable to all biological fluids. This method makes use of a PCO2-electrode, placed in a perspex reaction/measuring chamber, connected with a IL blood gas analyzer, type 413 equiped with an electronic data-ready indicator system. The advantages of the proposed method are: 1. Depending upon the accuracy required for sera, the sample volume can be reduced to 20 or 10 micron1.2. A high degree of accuracy is obtained depending upon the sample volume used; for sera we calculated variation coefficients of 2%, 1% and 0.8% using sample volumes of 10, 20 and 30 micron 1, respectively. 3. The proposed method is simple and requires only two minutes for one determination. 4. In a comparative study an almost perfect relationship was found between the proposed method and the indirect method by calculation with a constant pK1g value.

摘要

本文介绍了一种适用于所有生物体液的总二氧化碳含量测定的简单方法。该方法利用置于有机玻璃反应/测量室中的PCO2电极，与配备电子数据就绪指示系统的型号为413的IL血气分析仪相连。该方法的优点如下：1. 根据血清所需的准确度，样本体积可减少至20或10微升。2. 根据所用样本体积可获得高度的准确度；对于血清，分别使用10、20和30微升样本体积时，我们计算出的变异系数分别为2%、1%和0.8%。3. 该方法简单，一次测定仅需两分钟。4. 在一项对比研究中，通过使用恒定的pK1g值进行计算，发现该方法与间接法之间存在几乎完美的关系。