一种测定生物流体中总二氧化碳浓度的新参考方法。

A new reference method for the determination of the total CO2 concentration in biological fluids.

作者信息

Dijkhuizen P, Fongers T M, Rispens P, Zijlstra W G

出版信息

Clin Chim Acta. 1978 Jun 15;86(3):339-47. doi: 10.1016/0009-8981(78)90391-1.

Abstract

It appeared that a part of a measuring system recently developed for the determination of the oxygen content of blood (Dijkhuizen, P., Kwant, G. and Zijlstra, W.G. (1976) Clin. Chim. Acta 68, 79), was perfectly suitable for measuring the total CO2 content of blood, plasma or other fluids. CO2-free room air is pumped through an extraction vessel in which all the CO2 of the sample is set free by an acid reagent, and swept by the carrier gas to a titration vessel containing a BaCl2 solution. CO2 is bound as BaCO3 and the ensuing H+ titrated with an NaOH solution. The method was tested by measuring a series of Na2CO3 reference solutions. The values measured by titration amounted to 99.4 +/- 0.8% of the concentration of the reference solutions (range 10--50 mmol 1(-1). The coefficient of variation was 1.8% for 5 mmol 1(-1) solutions and 0.2% for 50 mmol 1(-1) solutions. In measuring a series of 60 blood samples the coefficient of variation as calculated from duplicate determinations was 1%.

摘要

最近开发的用于测定血液中氧含量的测量系统的一部分（Dijkhuizen, P., Kwant, G. 和 Zijlstra, W.G. (1976) Clin. Chim. Acta 68, 79），似乎非常适合测量血液、血浆或其他液体中的总二氧化碳含量。不含二氧化碳的室内空气被泵入一个萃取容器，样品中的所有二氧化碳通过一种酸性试剂被释放出来，并被载气吹扫到一个装有氯化钡溶液的滴定容器中。二氧化碳以碳酸钡的形式结合，随后用氢氧化钠溶液滴定产生的氢离子。通过测量一系列碳酸钠标准溶液对该方法进行了测试。滴定测得的值相当于标准溶液浓度的99.4±0.8%（范围为10--50 mmol 1(-1)）。对于5 mmol 1(-1)的溶液，变异系数为1.8%，对于50 mmol 1(-1)的溶液，变异系数为0.2%。在测量一系列60个血液样本时，由重复测定计算得出的变异系数为1%。