Andersen K J, Romslo I
Clin Chim Acta. 1977 Apr 15;76(2):219-22. doi: 10.1016/0009-8981(77)90099-7.
The effect of conjugated bile acids and detergents on the radiosorbent technique for the determination of vitamin B-12 activity is reported. It is shown that whereas the non-ionic detergent Triton X-100 has no effect on the vitamin B-12-radiosorbent assay, the addition of ionic detergents (e.g. glycocholic acid, taurocholic acid or sodium laurly sulfate) results in a falsely elevated vitamin B-12 activity presumably due to the disruption of the binding of vitamin B-12 to the intrinsic factor-Sephadex complex. This effect may be of importance not only to the radiosorbent assaying of vitamin B-12, but to the in vivo intestinal absorption of vitamin B-12 as well.
报道了结合胆汁酸和去污剂对用于测定维生素B - 12活性的放射吸附技术的影响。结果表明，非离子去污剂吐温X - 100对维生素B - 12放射吸附测定无影响，而加入离子去污剂（如甘氨胆酸、牛磺胆酸或月桂基硫酸钠）会导致维生素B - 12活性假性升高，这可能是由于维生素B - 12与内因子 - 葡聚糖凝胶复合物的结合被破坏所致。这种影响不仅对维生素B - 12的放射吸附测定很重要，对维生素B - 12在体内的肠道吸收也很重要。
