Kubota S, Siomi H, Hatanaka M, Pomerantz R J
Dorrance H Hamilton Laboratories, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
Oncogene. 1996 Apr 4;12(7):1441-7.
The MT-2 cell-line, which had been established through in vitro cell to cell transmission of human T-cell leukemia virus type I (HTLV-I) among human primary lymphocytes, was shown to possess multiple copies of integrated proviruses, including defective proviral genomes. By analysing a genomic clone, we identified the integration site of a single HTLV-I long terminal repeat (LTR) in the interleukin-9 (IL-9) receptor (IL-9R) gene. The integrated HTLV-I-LTR was shown to be functional as a promoter and the integration site was located in an intron upstream of the first coding exon of the IL-9R gene. Upon analysis of total cellular RNA, specific expression of HTLV-I-LTR Il-9R chimeric mRNAs in MT-2 cells was demonstrated. Cloning and characterization of these cDNAs have identified HTLV-I-IL-9R chimeric splicing, using either intact or alternative splice sites within the IL-9R gene. The potential roles of multiple interactions between IL-9, IL-9R and HTLV-I in the monoclonal expansion and transformation of MT-2 cells are explored.
MT-2细胞系是通过人T细胞白血病病毒I型（HTLV-I）在人原代淋巴细胞间进行体外细胞到细胞的传播而建立的，该细胞系被证明拥有多个整合前病毒拷贝，包括缺陷前病毒基因组。通过分析一个基因组克隆，我们确定了白细胞介素9（IL-9）受体（IL-9R）基因中单个HTLV-I长末端重复序列（LTR）的整合位点。整合的HTLV-I-LTR被证明具有启动子功能，且整合位点位于IL-9R基因第一个编码外显子上游的一个内含子中。在对总细胞RNA进行分析时，证实了MT-2细胞中HTLV-I-LTR Il-9R嵌合mRNA的特异性表达。对这些cDNA的克隆和特性分析确定了HTLV-I-IL-9R嵌合剪接，其使用了IL-9R基因内完整或替代的剪接位点。我们探讨了IL-9、IL-9R和HTLV-I之间多种相互作用在MT-2细胞单克隆扩增和转化中的潜在作用。
