人卵泡液和小鼠卵丘细胞协同作用可增强植入前小鼠Balb/cJ胚胎的发育。
Human follicular fluid and mouse cumulus cells act synergistically to enhance preimplantation mouse Balb/cJ embryo development.
作者信息
Roudebush W E, Levine A S, Lodge J S, Tsai C C, Butler W J
机构信息
Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425-2233, USA.
出版信息
J Assist Reprod Genet. 1995 Nov;12(10):733-7. doi: 10.1007/BF02212902.
PROBLEM
The development of preimplantation mammalian embryos in vitro is less than optimal. Follicular fluid and cumulus cells have both been used, independently, to improve preimplantation embryo quality in culture.
METHOD
To determine the ability of mouse cumulus cell coculture in the presence of human follicular fluids to support preimplantation mouse Balb/cJ embryo development in vitro.
RESULTS
Culture of preimplantation mouse Balb/cJ embryo's independently in human follicular fluid or on mouse cumulus cells had no significant affect on blastocyst. The coculture of mouse Balb/cJ preimplantation-stage embryos on mouse cumulus cells in the presence of human follicular fluid significantly (P < 0.01) improved blastocyst development and the total number of cells per blastocyst.
CONCLUSION
Cumulus cells and follicular fluid have a positive synergistic affect on preimplantation mouse Balb/cJ embryo development and formation in vitro.
问题
哺乳动物植入前胚胎在体外的发育情况不尽人意。卵泡液和卵丘细胞曾被单独用于提高培养中植入前胚胎的质量。
方法
为了确定在人卵泡液存在的情况下，小鼠卵丘细胞共培养支持体外植入前小鼠Balb/cJ胚胎发育的能力。
结果
将植入前小鼠Balb/cJ胚胎单独培养于人卵泡液中或培养于小鼠卵丘细胞上，对囊胚没有显著影响。在人卵泡液存在的情况下，将小鼠Balb/cJ植入前阶段胚胎与小鼠卵丘细胞共培养，显著（P < 0.01）改善了囊胚发育及每个囊胚的细胞总数。
结论
卵丘细胞和卵泡液对体外植入前小鼠Balb/cJ胚胎的发育和形成具有积极的协同作用。
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