Macho L, Ficková M, Zórad S
Ustav experimentálnej endokrinológie, Slovenskej akadémie vied v Bratislave.
Bratisl Lek Listy. 1995 Nov;96(11):581-5.
Underfeeding or overfeeding of rats during the suckling period, realized by the adjustment of the number of sucklings per litter, are followed by important changes in the function of endocrine glands and hormonal stimulation of lipolysis in adipocytes. For explanation of causes of the differences in the stimulation of lipolysis the changes of beta-receptors in adipocytes were studied in animals with different neonatal nutrition. The number of suckling pups in litter was adjusted to 4, 8, 14 on the second postnatal day. After weaning (at the age of 30-500 days) the size of adipocytes, basal and norepinephrine stimulated lipolysis, the binding of 3H-dihydroalprenolol in adipocytes were determined. In neonatally underfed animals, in comparison to overfed, those a decrease in the size of adipocytes, a diminution of basal lipolysis and lower stimulation of lipolysis by norepinephrine were observed. The decrease in binding capacity (at the age of 30-60 days) and of affinity of beta-receptors (at the age of 60 to 360 days) were found in neonatally overfed rats. These results showed that the changes of beta-adrenergic receptors in adipocytes of animals with neonatal nutritions have a role in the alteration of the stimulation of lipolysis in adipocytes. (Tab. 2, Fig. 4, Ref. 29.).
通过调整每窝哺乳仔鼠的数量来实现哺乳期大鼠的喂养不足或过度喂养，这会导致内分泌腺功能以及脂肪细胞中脂肪分解的激素刺激发生重要变化。为了解释脂肪分解刺激差异的原因，对不同新生儿营养状况的动物脂肪细胞中的β受体变化进行了研究。在出生后第二天，将每窝哺乳幼崽的数量调整为4只、8只、14只。断奶后（30 - 500日龄），测定脂肪细胞大小、基础和去甲肾上腺素刺激的脂肪分解以及脂肪细胞中3H - 二氢阿普洛尔的结合情况。与过度喂养的动物相比，新生儿期喂养不足的动物脂肪细胞大小减小，基础脂肪分解减少，去甲肾上腺素对脂肪分解的刺激降低。在新生儿期过度喂养的大鼠中发现结合能力（30 - 60日龄时）和β受体亲和力（60至360日龄时）下降。这些结果表明，新生儿营养状况不同的动物脂肪细胞中β - 肾上腺素能受体的变化在脂肪细胞脂肪分解刺激的改变中起作用。（表2，图4，参考文献29）
