Nakayama T, Hashimoto S, Amemiya E, Horie T
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Clin Exp Immunol. 1996 May;104(2):318-24. doi: 10.1046/j.1365-2249.1996.13702.x.
Two types of receptor for tumour necrosis factor-alpha (TNF-R), the 55-kD receptor (TNF-RI) and the 75-kD receptor (TNF-RII), have been identified. Soluble TNF-RI (sTNF-RI) and soluble TNF-RII (sTNF-RII) can be measured in culture supernatants and biological fluids, and the role of sTNF-R has been suggested. In the present study, we measured plasma sTNF-RI and sTNF-RII levels in 19 patients with active sarcoidosis by ELISA in order to assess the state of both types of receptors in this disease. Both plasma sTNF-RI and sTNF-RII levels in patients with active sarcoidosis were significantly higher than those in normal control subjects. A longitudinal evaluation of plasma sTNF-RI and sTNF-RII levels showed that the magnitude of changes in sTNF-RII was closely related with the clinical course of sarcoidosis. These results suggest that plasma sTNF-RII levels may be useful parameters for monitoring the clinical course of sarcoidosis as well as markers for identifying disease activity.
已鉴定出两种肿瘤坏死因子-α（TNF-R）受体，即55-kD受体（TNF-RI）和75-kD受体（TNF-RII）。可在培养上清液和生物体液中检测可溶性TNF-RI（sTNF-RI）和可溶性TNF-RII（sTNF-RII），并且已表明sTNF-R的作用。在本研究中，我们通过酶联免疫吸附测定法（ELISA）测量了19例活动性结节病患者血浆中的sTNF-RI和sTNF-RII水平，以评估该疾病中两种受体的状态。活动性结节病患者的血浆sTNF-RI和sTNF-RII水平均显著高于正常对照受试者。对血浆sTNF-RI和sTNF-RII水平的纵向评估表明，sTNF-RII的变化幅度与结节病的临床病程密切相关。这些结果表明，血浆sTNF-RII水平可能是监测结节病临床病程的有用参数，也是识别疾病活动的标志物。
