Song S Y, Asakai R, Kenmotsu N, Taoka M, Isobe T, Yamakuni T
Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo 194, Japan.
Endocrinology. 1996 Apr;137(4):1423-8. doi: 10.1210/endo.137.4.8625920.
We examined the gene expression of V-1, a novel soluble protein with the cdc10/SWI6 motif, in pseudopregnant rat ovaries. Northern blot analysis on days 1, 5, and 11 of pseudopregnancy revealed an approximately 2-fold increase in the V-1 messenger RNA (mRNA) expression level on day 5 to that on day 1, and no significant change was observed between those on day 5 and day 11. An injection of human CG on days 5 further increased th V-1 mRNA level to about 1.6-fold of that of the untreated control. Western blot analysis showed higher V-1 protein expression on days 5 and 11 of pseudopregnancy than that on day 1. In situ hybridization and immunohistochemistry with ovaries on day 3 of pseudopregnancy showed that luteal cells of corpora lutea and also cells of the coexisting follicles including oocytes express V-1 mRNA and the protein, with apparent rank order of the expression: oocytes > luteal cells > follicular cells >> atretic follicular cells including oocytes. These data indicate the dynamic change in the V-1 gene expression in the ovarian steroidogenic cells and oocytes and suggest potential roles of the V-1 protein in ovarian functions including corpus luteum formation and folliculogenesis.
我们检测了一种具有cdc10/SWI6基序的新型可溶性蛋白V-1在假孕大鼠卵巢中的基因表达。对假孕第1天、第5天和第11天进行的Northern印迹分析显示，V-1信使核糖核酸（mRNA）表达水平在第5天比第1天增加了约2倍，而第5天和第11天之间未观察到显著变化。在第5天注射人绒毛膜促性腺激素（hCG）进一步使V-1 mRNA水平增加至未处理对照组的约1.6倍。蛋白质印迹分析显示，假孕第5天和第11天的V-1蛋白表达高于第1天。对假孕第3天的卵巢进行原位杂交和免疫组织化学分析表明，黄体的黄体细胞以及包括卵母细胞在内的共存卵泡的细胞均表达V-1 mRNA和蛋白，其表达的明显顺序为：卵母细胞＞黄体细胞＞卵泡细胞＞＞包括卵母细胞在内的闭锁卵泡细胞。这些数据表明卵巢类固醇生成细胞和卵母细胞中V-1基因表达的动态变化，并提示V-1蛋白在包括黄体形成和卵泡发生在内的卵巢功能中可能发挥的作用。
