Reiser H, Schneeberger E E
Division of Lymphocyte Biology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Eur J Immunol. 1996 Apr;26(4):880-5. doi: 10.1002/eji.1830260424.
We analyzed the expression and function of the co-stimulatory molecules B7-1 (CD80) and B7-2 (CD86) during contact sensitivity reactions induced by the hapten 2,4-dinitrofluorobenzene (DNFB). In the normal skin, only a few epidermal Langerhans cells or dermal dendritic cells express B7-2. In contrast, following challenge with DNFB, expression of B7-2 is up-regulated in both epidermis and dermis. Importantly, B7-1 is induced later and at lower levels compared to B7-2. Intravenous injections of anti-B7-2 mAb, but not anti-B7-1 mAb partially inhibit the hapten-induced contact sensitivity reaction. Experiments in which mice are injected differentially with anti-B7-2 mAb, either before the afferent or before the efferent phase of the contact sensitivity response, suggest that B7-2 is important for successful antigen priming.
我们分析了在由半抗原2,4 -二硝基氟苯（DNFB）诱导的接触性敏感反应过程中，共刺激分子B7-1（CD80）和B7-2（CD86）的表达及功能。在正常皮肤中，仅有少数表皮朗格汉斯细胞或真皮树突状细胞表达B7-2。相比之下，用DNFB激发后，表皮和真皮中B7-2的表达均上调。重要的是，与B7-2相比，B7-1诱导较晚且水平较低。静脉注射抗B7-2单克隆抗体而非抗B7-1单克隆抗体可部分抑制半抗原诱导的接触性敏感反应。在接触性敏感反应的传入或传出阶段之前分别给小鼠注射抗B7-2单克隆抗体的实验表明，B7-2对于成功的抗原致敏很重要。
