Katayama I, Matsunaga T, Yokozeki H, Nishioka K
Department of Dermatology, Tokyo Medical and Dental University School of Medicine, Japan.
Br J Dermatol. 1997 Jun;136(6):846-52.
The hapten, trinitrobenzene sulphonic acid, induced weak B7-1 (CD80) and moderate B7-2 (CD86) expression on Langerhans cells and mRNA expression of both molecules in organ-cultured murine skin. The intradermal injection of hapten-treated epidermal cells induced hapten-specific contact sensitivity in synergic mice. Cells of the keratinocyte cell line, Pam 212, or epidermal cells treated with a mixture of anti-Ia/thy1.2/gamma delta antibody plus complement, did not show any sensitizing ability. When hapten-treated epidermal cells were injected into mice after incubation with anti-B7-2 (CD86) or B7-1 (CD80) antibody the resultant contact sensitivity reaction was decreased to less than 50% of the control reaction, a reduction which was similar to that seen with the anti-ICAM-1 and anti-LFA-1 antibody-induced inhibition of contact sensitivity. Anti-B7-1 (CD80) or anti-B7-2 (CD86) antibody also inhibited hapten-specific lymphocyte proliferation or the allogenic mixed lymphocyte and epidermal cell reaction in vitro, although the inhibitory effect of anti-B7-1 antibody was not as significant as that of anti-B7-2 antibody. These results indicate that costimulatory signals induced by a hapten on epidermal Langerhans cells play an important role in the induction of hapten-specific contact sensitivity in mice.
半抗原三硝基苯磺酸可诱导朗格汉斯细胞上B7-1（CD80）表达较弱而B7-2（CD86）表达中等，并可诱导器官培养的小鼠皮肤中这两种分子的mRNA表达。皮内注射经半抗原处理的表皮细胞可在协同小鼠中诱导半抗原特异性接触性超敏反应。角质形成细胞系Pam 212的细胞或用抗Ia/thy1.2/γδ抗体加补体的混合物处理的表皮细胞均未显示出任何致敏能力。当用抗B7-2（CD86）或B7-1（CD80）抗体孵育后将经半抗原处理的表皮细胞注射到小鼠体内时，所产生的接触性超敏反应降低至对照反应的50%以下，这种降低类似于抗ICAM-1和抗LFA-1抗体诱导的接触性超敏反应抑制。抗B7-1（CD80）或抗B7-2（CD86）抗体在体外也可抑制半抗原特异性淋巴细胞增殖或同种异体混合淋巴细胞与表皮细胞反应，尽管抗B7-1抗体的抑制作用不如抗B7-2抗体显著。这些结果表明，半抗原在表皮朗格汉斯细胞上诱导的共刺激信号在小鼠半抗原特异性接触性超敏反应的诱导中起重要作用。
